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Why pcr takes time
In this test, a swab is taken from the throat or nose of the person who is getting tested. This swab contains a small quantity of the RNA of the virus, therefore it is amplified to produce material that is enough for testing whether or not coronavirus has infected the person. To detect the same in the test, the RNA is converted into a two-strand DNA, using the process known as reverse transcription. Although this test is more accurate than a rapid antigen test, the time taken is also more.
The test can be completed in four to eight hours, however, the results are available in one day due to time taken in collection and in the transportation of samples to the labs. PCR was invented in the s and is now used in a variety of ways, including DNA fingerprinting, diagnosing genetic disorders and detecting bacteria or viruses. Because molecular and genetic analyses require significant amounts of a DNA sample, it is nearly impossible for researchers to study isolated pieces of genetic material without PCR amplification.
This method adds fluorescent dyes to the PCR process to measure the amount of genetic material in a sample. The testing process begins when healthcare workers collect samples using a nasal swab or saliva tube. The two DNA template strands are then separated. Primers attach to the end of these strands. After the primers attach, new complementary strands of DNA extend along the template strand. As this occurs, fluorescent dyes attach to the DNA, providing a marker of successful duplication.
At the end of the process, two identical copies of viral DNA are created. This means the sample is from an infected individual. The primers only amplify genetic material from the virus, so it is unlikely a sample will be positive if viral RNA is not present. If it does, it is called a false positive.
How Long Does It Take to Get COVID Results by Test Type?.
Image for representation. In this test, a swab is taken from the throat or nose of the person who is getting tested. This swab contains a small quantity of the RNA of the virus, therefore it is amplified to produce material that is enough for testing whether or not coronavirus has infected the person. To detect the same in the test, the RNA is converted into a two-strand DNA, using the process known as reverse transcription.
Although this test is more accurate than a rapid antigen test, the time taken is also more. The test can be completed in four to eight hours, however, the results are available in one day due to time taken in collection and in the transportation of samples to the labs.
This also adds to the time taken by labs to conduct this test. If the testing lab is far from the place where the sample of the person has been collected, the test result may be available after 48 hours or more, in some cases. On the other hand, the results of the rapid antigen test can come out in about half an hour but the accuracy is less than that of an RT PCR test.
The cost of RT PCR test in private labs has recently been reduced by the Delhi government and is now capped at Rs , if the sample is collected at the facility.
However, if a technician comes to your home for sample collection, the maximum price that can be taken for the same is Rs 1,
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